Component-resolved diagnosis of pollen allergy based on skin testing with profilin, polcalcin and lipid transfer protein pan-allergens
نویسندگان
چکیده
منابع مشابه
Component-resolved diagnosis of pollen allergy based on skin testing with profilin, polcalcin and lipid transfer protein pan-allergens.
BACKGROUND Allergy diagnosis needs to be improved in patients suffering from pollen polysensitization due to the existence of possible confounding factors in this type of patients. OBJECTIVE To evaluate new diagnostic strategies by comparing skin responses to pan-allergens and conventional allergenic extracts with specific IgE (sIgE) to purified allergen molecules. METHODS One thousand thre...
متن کاملUnderstanding patient sensitization profiles in complex pollen areas: a molecular epidemiological study.
BACKGROUND Allergy diagnosis in patients exposed to multiple pollen species is complex and misdiagnosis is often a cause for unsuccessful specific immunotherapy. OBJECTIVE We studied the sensitization profile of individual allergens (major, minor and pan-allergens) in pollen-sensitized patients in a region with high exposure to olive pollen by investigating the influence of minor allergens on...
متن کاملCharacterization of profilin and polcalcin panallergens from ash pollen.
BACKGROUND Ash (Fraxinus excelsior) is an important source of allergenic pollen in temperate areas of Europe. Profilin and polcalcin are 2 important panallergens involved in cross-reactivity between different sources. OBJECTIVE To clone and produce Fra e 2 (profilin) and Fra e 3 (polcalcin) as recombinant proteins and evaluate their immunological properties using the natural forms obtained fr...
متن کاملMicroarray based IgE detection in poly-sensitized allergic patients with suspected food allergy - an approach in four clinical cases.
BACKGROUND Component-resolved diagnosis and microarray technology have been recently introduced into clinical allergy practice, and may be particularly useful in poly-sensitized allergic patients. METHODS We compare the clinical usefulness of a microarray-based IgE detection assay (ISAC(®)) with skin tests and specific IgE with standard allergens (sIgE) or their monocomponents in four case re...
متن کاملAnalysis of the allergenic profile of patients hypersensitive to pollen pan-allergens living in two distinct areas of northern Italy.
BACKGROUND The allergenic profile of patients hypersensitive to pollen pan-allergens, profilin and polcalcin, has received little attention so far. OBJECTIVE To detect whether hypersensitivity to profilin and polcalcin follows sensitization to specific allergen sources or represents a primary phenomenon, and to examine the sensitization profiles of patients hypersensitive to pollen pan-allerg...
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ژورنال
عنوان ژورنال: Clinical & Experimental Allergy
سال: 2009
ISSN: 0954-7894,1365-2222
DOI: 10.1111/j.1365-2222.2009.03351.x